TFEB Knockout cell line (Hep G2)

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TFEB Knockout cell line (Hep G2) Order

TFEB Knockout cell line (Hep G2)
Catalog#
YKO-HT3769
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
TFEB Knockout cell line (Hep G2)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
TFEB Knockout cell line (Hep G2)
Species
Gene Name
TFEB
Gene ID
7942
Gene Information
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the TFEB gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, TFEB gene knockout TFEB Knockout cell line (Hep G2) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
TFEB Knockout cell line (Hep G2)
TFEB Knockout cell line (Hep G2)
Catalog#
YKO-HT3769
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order
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